The Effect Of Changing The Polarity Of Mobile Phase On...

PART A - HPLC METHOD DEVELOPMENT AND QUANTITATIVE ANALYSIS Aim-To investigates the effect of changing the polarity of mobile phase on Retention time (RT) and Column capacity (k’). Results- Chromatogram for 60% methanol Tm- 1.597 Tables- For Retention time of compounds for 60% and 30% methanol. Table 1- Compound tR Xanthine 1.777 3-methylxanthine 2.500 Theobromine 2.612 Theophyline 2.798 Caffeine 3.865 Table1. shows retention time of five different compounds for 60% methanol. Table 2- Compound tR Xanthine 2.518 3-methylxanthine 2.984 Theobromine 3.429 Theophyline 4.659 Caffeine 6.615 Table2. shows retention time of five different compounds for 30% methanol. Tm of caffeine from caffeine standard chromatogram = 1.560 TR of caffeine from caffeine standard chromatogram = 6.669 Calculations- For 60% methanol k’ = ((tR-tM))/tM , here tR changes for each compound but tM remains same. k’ for Xanthine = ((1.777-1.597))/1.597 = 0.133 k’ for 3-methylxanthine = 0.565 k’ for theobromine = 0.635 k’ for theophylline = 0.752 k’ for caffeine = 1.420 For 30% methanol – k’ = ((tR-tM))/tM , here tR changes for each compound but tM remains same. k’ for Xanthine= ((2.518-1.560))/1.560 = 0.614 k’ for 3-mehtylaxanthine= 0.912 k’ for Theobromine= 1.198 k’ for Theophylline= 1.986 k’ for Caffeine= 3.240 For Caffeine standard chromatogram K’ for Caffeine =((6.669-1.560))/1.560 = 3.275 Discussion- The results were found and k’ was calculated for 60% and 30% methanol HPLCShow MoreRelatedChromatography Is Common And Useful Separation Technique Essay2245 Words   |  9 PagesmL above the top sand layer and then transferred the sample onto the column. Added 2 mL of hexanes to the column and allowed the solvent to drain to just slightly above the sand at the top of the packed column. Repeated, adding 2 mL of hexanes at a time, until the sample was loaded onto the column. Replaced waste beaker with pre-weighed flask #1. Began using 2% ether/hexane solution to flush fluorene through column. Used air pressure to speed rate of solvent flow through column. Continued flushingRead MoreThin Layer Chromatography1903 Words   |  8 PagesExperiment 5 Title : Thin Layer Chromatography Objectives: i. To distinguish polar and non-polar solvents. ii. To familiar with the analysis technique by using the thin layer chromatography. iii. To differentiate the retention factor, Rf for different compounds. [pic] Result: |Compound |Distance traveled by the compound | |o-nitroanaline |2.45 Read MoreData Storage Using Nano-Technology and Electronics5235 Words   |  21 Pagesway to create permanent (non-volatile) data storage using electronic applications. Trends in data storage: International Data Corporation estimated that the total amount of digital data was 281 billion gigabytes in 2007, and had for the first time exceeded the amount of storage. Before moving any further in understanding emerging trends in electronic storage, we here briefly describe an EEPROM and then comprehensively cover a few advanced non-volatile data storage devices. Read MoreSexually Transmitted Diseases35655 Words   |  143 Pages 148-150 Onion and cheek cells 150-151 Ecological study 154 Seed structure and germination 155-157 Reproduction in plants 158-165 Sexually transmitted diseases 166-176 Birth Control and contraceptives 177-185 Effects on population growth 186-187 Drug and drug abuse 188-191 Man’s impact on the environment 192-199 Reproduction in man 200-201 References 202 Introduction Many people live their lives without having a clue about certainRead MoreRetailing and Big Bazaar12258 Words   |  50 Pagesthe award of degree of Master of Business Administration submitted to College Name. The results embodied in this thesis have not been submitted to any other University or Institution for the award of any Degree/Diploma Certificate or Published any time before. Place: Date (YOUR NAME) ROLL NO ACKNOWLEDGEMENT I am thankful to NAME (AsstRead MoreRastafarian79520 Words   |  319 PagesSelassie to remove the Italians from his homeland of Ethiopia, which became the ï ¬ rst African nation to effectively oust, by force, a colonial power. These were monumental times, and these men, fully steeped in the apocalyptic visions of the world, saw something important in all of these happenings. I grew up in Jamaica at a time when Rastas were still regarded as useless, lazy, half-insane, ganja-smoking illiterates who were of no value to society. Teachers, students, ofï ¬ ce workers, and anyone of